Research, education, management

Professional summary

• 16+ years teaching and researching in molecular and cell biology, RNA biology, medical parasitology, physiology and immunology. The work led to five publications and seven posters presented at national and international conferences.
• DNA, RNA and protein extraction from several tissue types, electrophoresis, detection and quantification, miRNA techniques, qPCR, western blot, ELISA, HPLC, cell culture (microalgae, mammalian cell lines, primary CD4 T cells), T cell proliferation assays, cell transfection, monoclonal antibody production, flow cytometry, bioinformatics, computational modelling, R, data analysis, lab management.
• Fast learner, goal-driven, organised. Project and resource management, market and product knowledge, interpersonal and written communication, working independently and collaboratively, creative and strategic thinking, problem solving, reporting, multitasking, presenting, training and teaching.

Work experience

• 2019 – present Teacher and tutor of biology and sciences
  Freelance, Lisbon area — online and in person Subjects taught, in Portuguese and English →
• 2022 – 2023 Science teacher
  Grémio de Instrução Liberal de Campo de Ourique Middle school, ages 12–14: Ciências Naturais and Físico-Química.
• 2019 – 2021 Teacher of IGCSE and A Level Biology
  Sapienti Unum Lda., Cascais Cambridge international school. Online and in-person classes.
• 2015 – 2019 T cell biologist — PhD candidate in Systems Biology
  BioISI, University of Lisbon Human helper T cell differentiation and TCR signalling; RNA biology, transcriptomics and logical modelling.
• 2013 – 2014 Research assistant in medical parasitology
  Institute for Hygiene and Tropical Medicine, New University of Lisbon
• 2011 – 2012 Research scientist, molecular physiology of microalgae
  BioISI, University of Lisbon
• 2010 – 2011 Research intern in microalgae photosynthesis
  Plant Biotechnology Lab, University of Lisbon
• 2009 Coastal manager
  City Hall of Cascais, Portugal
• 2007 Research intern in molecular biology and ovary development
  Centre of Marine Sciences, Faro, Portugal

Education

• 2009 – 2011 MSc, Cellular Biology and Biotechnology
  University of Lisbon, Portugal
• 2005 – 2007 MSc, Marine Biology — Marine Biotechnology
  University of the Algarve, Faro, Portugal
• 2002 – 2005 Licentiate, Marine Biology
  University of the Algarve, Faro, Portugal
• 2002 Secondary school, Science and Nature
  Escola Salesiana de Manique, Cascais, Portugal

Certificates — Portuguese originals and English translations (PDF) →

Certifications

• 2024 Google Data Analytics
  Google, via Coursera
• 2023 Google Project Management
  Google, via Coursera

Skills

Publications

• Antunes S., Ferrolho J., Domingues N., Santos A.S. et al. Anaplasma marginale and Theileria annulata in questing ticks from Portugal Experimental and Applied Acarology, 2016; 70(1):79–88
• Ferrolho J., Domingues N., Domingos A., Santos-Gomes G. The role of the regulatory CD4+CD25+ T cell subset in host homeostasis during protozoan infection International Trends in Immunity, 2015; 3(1):6–16
• Antunes S., Merino O., Lérias J., Domingues N. et al. Artificial feeding of Rhipicephalus microplus female ticks with anti-calreticulin serum does not influence tick and Babesia bigemina acquisition Ticks and Tick-Borne Diseases, 2015
• Cartaxana P., Domingues N., Cruz S., Jesus B. et al. Photoinhibition in benthic diatom assemblages under light stress Aquatic Microbial Ecology, 2013; 70:87–92
• Domingues N., Cartaxana P., Matos A.R., Marques da Silva J. Response of the diatom Phaeodactylum tricornutum to photooxidative stress resulting from high light exposure PLoS ONE, 2012; 7(6):e38162

Conferences

• Poster — Domingues N., Pinto F., Espada de Sousa A., Gama-Carvalho M. Exploring miR-34c-5p modulation of effector CD4 T cell differentiation through logical modelling 2018–2019 · 44th Portuguese Society of Immunology Meeting (IMM, Lisbon) · 5th European Congress of Immunology (RAI, Amsterdam) · 8th ptRNA meeting (i3S, Porto)
• 2nd Meeting in Mathematics and Biology 2016 · i3S, Porto, Portugal
• Posters — What are Portuguese ticks hiding? and Artificial feeding of Rhipicephalus microplus female ticks: effects of serum enriched with anti-calreticulin antibodies on tick and Babesia bigemina acquisition 2014 · 12th Biennial Conference of the Society for Tropical Veterinary Medicine and 8th International Conference on Ticks and Tick-borne Pathogens, Cape Town, South Africa
• Poster — Deloffre L., Domingues N., Canário A. Characterization of BMP receptor IB in Oreochromis mossambicus ovary — gene expression during follicle growth 2008 · 6th International Symposium in Fish Endocrinology, Calgary, Canada
• Poster — Domingues N., Deloffre L., Canário A. Gene expression during oogenesis in the Mozambique tilapia (Oreochromis mossambicus) 2007 · 6th Iberian Congress on Compared Endocrinology, Cadiz, Spain

Also worth mentioning

• 2022 Completed the Piscine C-language bootcamp at 42 Lisboa.
• 2020 Cambridge teaching courses Introductory AS & A Level Biology (9700) · IGCSE Biology (0610) · AS & A Level Marine Science (9693) · Marking Workshop, AS & A Level Biology — Cambridge Assessment International Education
• 2015 – 2019 Voluntary work at CheckpointLX, GAT, Lisbon.
• 2018 Travel grant for the 5th European Congress of Immunology, Amsterdam.
• 2018 Co-organised the workshop Integrative Approaches in Neurodegeneration, University of Lisbon.
• 2016 – 2018 Soft-skill courses at the University of Lisbon: scientific writing, entrepreneurship, intellectual and industrial property, ethics in science and bioethics.
• 2016 Volunteer at Web Summit — participant welcoming and registration, Lisbon.
• 2014 Co-organised the workshop “Vectors of human and animal diseases: from epidemiology to control”, Faculty of Veterinary Sciences, University of Lisbon.
• 2014 Training school at ANSES, Paris (COST travel grant): qPCR on chips for high-throughput detection of vector-borne pathogens.
• 2013 Multimedia Design and Communication, Academic Profession, 1st semester, Copenhagen School of Design and Technology, Denmark.
• 2010 1st prize, 9th Conference of Entrepreneurship and Business Creation, Audax/ISCTE-IUL Portugal Start Up. Group business project on extracting pectin from juice-industry residues such as apple pomace.
• 1996 – 2014 Topography work at Platerra Lda. and freelance — computer and field work across Portugal.
• 1990 – 2002 Competitive swimming, equestrianism, volleyball.
• Others Driving licence B. Cooking, healthy lifestyle and fitness, strength training, tech and videogames.